# Acartia tonsa mortality via neutral red vital staining of vertical net tows and associated CTD information # Collected on 5 day trips to mid-Chesapeake Bay, Aug-Oct 2013 # M. Roman; J. Pierson; C. Fitzgerald (UMCES) # version: 2018-01-12 # ========================= cruise date ------------------------- 1 2013-08-02 ========================= time_EDT EDT_DOY Station Lateral_station Sample_num Low_depth High_depth Genus Species Stage Alive Dead Percent_live Percent_dead Photo_files Comments Latitude Longitude Year Month CTD_Day GMT_Hour Minute Second GMT_DOY prSM t090C c0S_m sbeox0V wetStar obs bat xmiss sbeox0Mg_L sbeox0PS sigma_t00 depSM sal00 nbin flag ------------------------- 1150 214.49 MB M2 1 20 10 Acartia tonsa Nauplius 1 3 25 75 nd nd 38.46993 -76.38862 2013 8 2 14 46 31 214.62 15.11 17.19 3.6902 0.6149 2.5838 1.4335 3.4522 70.8245 0.3547 4.3206 20.1226 15 28.02 3.43 0 1150 214.49 MB M2 1 20 10 Acartia tonsa Egg 22 13 63 37 nd nd 38.46993 -76.38862 2013 8 2 14 46 31 214.62 15.11 17.19 3.6902 0.6149 2.5838 1.4335 3.4522 70.8245 0.3547 4.3206 20.1226 15 28.02 3.43 0 1157 214.50 MB M2 2 10 0 Acartia tonsa Adult 7 3 70 30 2013_08_05_018 and 2013_08_05_019 Unknown copepod: 1 live 38.46993 -76.38862 2013 8 2 14 46 31 214.62 5.29 17.49 2.5265 2.3710 15.3190 1.4109 16.3883 57.4912 6.9308 80.6663 12.6424 5.25 18.30 3.15 0 1157 214.50 MB M2 2 10 0 Acartia tonsa Copepodite 40 33 55 45 nd Possible count inaccuracies due to late identification of Oithona; some 38.46993 -76.38862 2013 8 2 14 46 31 214.62 5.29 17.49 2.5265 2.3710 15.3190 1.4109 16.3883 57.4912 6.9308 80.6663 12.6424 5.25 18.30 3.15 0 1157 214.50 MB M2 2 10 0 Acartia tonsa Nauplius 62 235 21 79 nd of the copepodite Acartia may actually be Oithona. Eggs and Nauplii are fine though 38.46993 -76.38862 2013 8 2 14 46 31 214.62 5.29 17.49 2.5265 2.3710 15.3190 1.4109 16.3883 57.4912 6.9308 80.6663 12.6424 5.25 18.30 3.15 0 1157 214.50 MB M2 2 10 0 Acartia tonsa Egg 39 7 85 15 nd nd 38.46993 -76.38862 2013 8 2 14 46 31 214.62 5.29 17.49 2.5265 2.3710 15.3190 1.4109 16.3883 57.4912 6.9308 80.6663 12.6424 5.25 18.30 3.15 0 1157 214.50 MB M2 2 10 0 Oithona sp. nd 21 40 34 66 nd nd 38.46993 -76.38862 2013 8 2 14 46 31 214.62 5.29 17.49 2.5265 2.3710 15.3190 1.4109 16.3883 57.4912 6.9308 80.6663 12.6424 5.25 18.30 3.15 0 1218 214.51 M3 NaN 3 10 0 Acartia tonsa Adult 5 0 100 0 subsample 1: 2013_08_06_020 and 2013_08_06_021 Likely some of sample lost during collection due to leaky vacuum filtration system 38.46082 -76.41212 2013 8 2 16 8 53 214.67 5.29 17.77 2.4915 2.5616 23.7142 1.6923 25.2918 49.1376 7.6155 89.1165 12.2742 5.25 17.90 3.25 0 1218 214.51 M3 NaN 3 10 0 Acartia tonsa Copepodite 10 12 45 55 subsample 2: 2013_08_06_022 and 2013_08_06_023 Suggest nauplii stages 1-4/5 don't take up the stain; don't stain clearly; 38.46082 -76.41212 2013 8 2 16 8 53 214.67 5.29 17.77 2.4915 2.5616 23.7142 1.6923 25.2918 49.1376 7.6155 89.1165 12.2742 5.25 17.90 3.25 0 1218 214.51 M3 NaN 3 10 0 Acartia tonsa Nauplius 22 169 12 88 nd stain is shielded from acidic conditions; or stain leeches out during storage; 38.46082 -76.41212 2013 8 2 16 8 53 214.67 5.29 17.77 2.4915 2.5616 23.7142 1.6923 25.2918 49.1376 7.6155 89.1165 12.2742 5.25 17.90 3.25 0 1218 214.51 M3 NaN 3 10 0 Acartia tonsa Egg 32 8 80 20 nd all small nauplii always fall into "dead" category. 38.46082 -76.41212 2013 8 2 16 8 53 214.67 5.29 17.77 2.4915 2.5616 23.7142 1.6923 25.2918 49.1376 7.6155 89.1165 12.2742 5.25 17.90 3.25 0 1218 214.51 M3 NaN 3 10 0 Oithona sp. nd 1 1 50 50 nd nd 38.46082 -76.41212 2013 8 2 16 8 53 214.67 5.29 17.77 2.4915 2.5616 23.7142 1.6923 25.2918 49.1376 7.6155 89.1165 12.2742 5.25 17.90 3.25 0 1401 214.58 M7 M3 4 5 0 Acartia tonsa Copepodite 12 1 92 8 subsample 2: 2013_08_06_026 and 2013_08_06_028 Sample overstained; combined with darkened room this helped distinguish 38.48245 -76.35638 2013 8 2 17 47 47 214.74 0.50 19.12 2.5183 2.9580 -2.3668 3.4191 140.1600 -1.9366 9.1685 109.9300 11.6940 0.5 17.53 2.00 0 1401 214.58 M7 M3 4 5 0 Acartia tonsa Nauplius 38 35 52 48 nd nauplii in stages 3-5. Very small nauplii still all appeared dead. 38.48245 -76.35638 2013 8 2 17 47 47 214.74 0.50 19.12 2.5183 2.9580 -2.3668 3.4191 140.1600 -1.9366 9.1685 109.9300 11.6940 0.5 17.53 2.00 0 1401 214.58 M7 M3 4 5 0 Acartia tonsa Egg 12 1 92 8 nd Sample dominated by debris. 38.48245 -76.35638 2013 8 2 17 47 47 214.74 0.50 19.12 2.5183 2.9580 -2.3668 3.4191 140.1600 -1.9366 9.1685 109.9300 11.6940 0.5 17.53 2.00 0